AAEI ENDONUCLEASE Meaning and
Definition
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AaeI endonuclease is a type II restriction enzyme that is obtained from five Methyladenine-specific modification and cleavage enzymes in the Arthrobacter aurescens strain TC1. It is classified under the family of restriction enzymes known as the EcoRI-like enzymes. These enzymes are primarily responsible for recognizing and cutting DNA sequences that contain adenine methylation, resulting in the formation of DNA fragments.
The AaeI endonuclease specifically recognizes a palindromic DNA sequence, 5'-A^pyPuTC-3', where 'py' represents methylated adenine and 'Pu' represents any purine nucleotide. Upon sensing the presence of this specific DNA sequence, AaeI endonuclease catalyzes the hydrolysis of the DNA phosphodiester backbone, leading to the cleavage of the DNA chain at specific points within the recognition sequence.
The endonuclease exhibits both recognition and cleavage activities, as it possesses two distinct catalytic domains. The first is the DNA-binding domain, which recognizes and binds to the specific DNA sequence, ensuring accurate positioning for cleavage. The second domain is the active nuclease domain, responsible for the enzymatic cleavage of the recognition sequence.
AaeI endonuclease, like many restriction enzymes, finds usage in various molecular biology applications, including DNA manipulation, cloning, and genetic engineering. It provides a valuable tool for DNA researchers to selectively cleave DNA at specific sites and facilitate the study and modification of DNA sequences.
Common Misspellings for AAEI ENDONUCLEASE
- zaei endonuclease
- saei endonuclease
- waei endonuclease
- qaei endonuclease
- azei endonuclease
- asei endonuclease
- awei endonuclease
- aqei endonuclease
- aawi endonuclease
- aari endonuclease
- aa4i endonuclease
- aa3i endonuclease
- aaeu endonuclease
- aaej endonuclease
- aaek endonuclease
- aaeo endonuclease
- aae9 endonuclease
- aae8 endonuclease
- aaei wndonuclease
- aaei sndonuclease
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